Pharmacy and Biomedical Sciences

Live Cell Imaging Microscope

Live Cell Imaging allows imaging of cells under physiological conditions. Time-lapse movies can be made from the images to allow the study of cell migration and invasion, for example, which are invaluable for our brain cancer research work.

Our facility consists of a Carl Zeiss AxioVert 200M inverted microscope housed in an incubator (temperature, humidity and CO2 controlled). Brightfield, phase, DIC and fluorescence imaging is possible. An Optigrid feature offers a degree of confocality. Objectives range from 5x to 100x and samples can be in slides, petri dishes, or more suitably, 24-well plates that allow numerous conditions to be investigated simultaneously. Specialist software (Volocity, Perkin Elmer) can also be used offline for image processing.

Contacts: Dr James Smith, Professor Geoff Pilkington, Jill Rice