Institute of Biomedical and Biomolecular Science (IBBS)

Molecular and genetic medicine

Techniques

In our studies we use:

  • Molecular biology techniques
  • Cell culture (established and primary cell cultures, cell transfection)
  • Immunolocalisation and visualisation techniques(confocal and video microscopy)
  • Biochemical/proteomics methods

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Fig.1

(A) Confocal co-localisation of alpha-dystrobrevin in testicular Sertoli cells (green) with collagen IV (purple) and nuclear countertsaining (white). (B) alpha-dystrobrevin co-localisation in the retinal glia (blue) surrounding blood vessels  (purple). (C) RT-PCR analysis of the alternative splicing of alpha-dystrobrevin transcripts in non-muscle tissues. (For details see: Lien et al, Cell Tissue Res. 2006). (D) Immuno co-localisation of alpha-DB (red) with GFAP (green) in glioma brain tumour cells in culture.

Cultured muscle cells contracting