Institute of Biomedical and Biomolecular Science (IBBS)

University of Portsmouth Imaging Centre (UPIC)

Facilities

Atomic-Force-Microscopy-(AFM)

Atomic Force Microscopy (AFM)

Digital Instruments/Veeco MultiMode/Nanoscope IV, encompassing TappingMode/Contact Mode; Force Spectroscopy, Chemical Force Microscopy (CFM), Magnetic Force Microscopy (MFM), Scanning Tunnelling Microscopy (STM).

Confocal-Laser-Scanning-Microscopy-(CLSM)

Confocal Laser Scanning Microscopy (CLSM)

Carl Zeiss LSM 710. Imager.Z2. Lasers: 405 nm, 453 nm, 458 nm, 488 nm, 514 nm, 633 nm.
Confocal-Laser-Scanning-Microscopy-(CLSM)-2

Confocal Laser Scanning Microscopy (CLSM)

Carl Zeiss LSM 510. AxioPlan 2 with AxioCam HRc camera. Lasers: 488 nm, 543 nm, 633 nm.
Calcium-Imaging-Confocal-Laser-Scanning-Microscopy

Calcium Imaging Confocal Laser Scanning Microscopy

Carl Zeiss Axioskop 2 with LSM 5 Pascal Exciter

Calcium-Imaging-Microscopy

Calcium Imaging Microscopy

Olympus BX51W1

Live-Cell-Imaging

Live Cell Imaging

Carl Zeiss Axiovert 200M (inverted) with incubator (temp, humidity and CO2 control); brightfield, phase, DIC and fluorescence; optigrid; x5 - x100 objectives; Volocity 5.2 software (Improvision).

Total-Internal-Reflectance-Microscopy-(TIRF)

Total Internal Reflectance Microscopy (TIRF)

Carl Zeiss Axiovert 200M (inverted) with laser (488 nm); brightfield, phase, DIC and fluorescence; microinjection; ´5 - ´100 objectives; AxioVision 4.7.1 software (Carl Zeiss).
Laser-Capture-Micro-Dissection-(LCMD)

Laser Capture Micro Dissection (LCMD)

Arcturus Varitas Microdissection Instrument (Model 704: IR capture laser and UV cutting laser with epi-fluorescence) for removing individual cells or multi-cellular structures tissue sections.
Scanning-Electron-Microscopy-(SEM)

Scanning Electron Microscopy (SEM)

Variable vacuum instrument (JEOL JSM-6060LV); samples can be imaged without vacuum.
Fluorescence-and-Light-Microscope

Fluorescence and Light Microscope

Zeiss AxioImager (with Qimaging Micropublisher brightfield colour camera).
Fluorescence-and-Light-Microscope-2

Fluorescence and Light Microscope

Olympus (1X71 inverted microscope).
Image-Analysis-Workstations

Image Analysis Workstations

with Volocity 5.2 (Improvision), AxioVision 4.7.1 software (Carl Zeiss) and various AFM image processing software (NanoScope, WSxM, SPIP, Gwyddion and in-house software).