School of Biological Sciences
Dr Graham Paul Malyon
- Qualifications: BSc (Hons) Marine Biology, PgCert Scientific Research Methods, PhD Biology
- Role Title: Lead Technician
- Address: Institute of Marine Sciences Laboratories, Langstone Harbour, Ferry Road, Eastney, Portsmouth, P04 9LY
- Telephone: 023 9284 5809
- Email: email@example.com
- Department: School of Biological Sciences
- Faculty: Faculty of Science
I am a marine biologist with current experience of molecular biology and biochemistry. The model organism used throughout my research is the wood-boring marine crustacean Limnoria quadripunctata. The focus of my research has been to discover the process by which L. quadripunctata digests lignocellulose with the view to develop novel mechanisms for advancing the efficiency of second generation biofules. This research has been driven by my desire to understand digestion in L. quadripunctata from the whole organism all the way down to the control of gene regulation, an approach that has been enhanced by the collection of direct evidence of changes to the lignocellulosic biomass on which it feeds.
Light microscopy, scanning electron microscopy, sectioning, fixation and dissection of tissue samples, preparation of biological samples for X-ray tomography, bacteriological assays, use of microelectrodes for the measurement of pH and oxygen. Experimental design and feeding assessment under laboratory conditions, use of computer programmes to provide automated solutions to counting procedures, design of tidal tanks, vacuum impregnation of wood with various compounds and solvents, RNA extraction for 454 transcriptome sequencing. Native PAGE and analysis of MALDI TOF proteomic data, In situ hybridisation of mRNA to whole mount tissue, Western blots, CMC enzyme assays and specific sugar enzyme activity assays combined with phenol oxidase assays for specific substrates. Wood timber durability trials, including the effect of wood extractives and physical properties of wood to durability to borer attack, the analysis of wood using X-ray diffraction and FT-IR ATR. Crystalline cellulose feeding assays, wood compositional analysis using HPLC and metabolomic analysis of tissue extract using NMR spectroscopy to track degradation products using 13C labelled wood. Chromatin immune precipitation of Limnoria genes related to feeding.