Funding

Self-funded PhD students only

Project code

PHBM4751019

Faculty

School of Pharmacy and Biomedical Sciences

Start dates

February and October

Closing date

Open all year round

This is a self-funded, 3 year full-time or 6 year part-time PhD studentship, to commence in October 2019 or February 2020. The project is supervised by Dr Qian An.

Breast cancer, along with lung cancer and melanoma, is one of the most aggressive human cancers in terms of developing brain metastasis, a condition which typically has a very poor prognosis. The negativity of the prognosis is mainly due to the presence of the blood–brain barrier (B-BB) that prevents a large number of antineoplastic agents from entering the brain.

However, previously published data has shown that tricyclic antidepressants (TCAs) can induce apoptosis (cell death) by targeting mitochondria in glioma, metastatic melanoma, lung and colorectal cancer cells. To date, the anti-cancer activity of TCAs in breast cancer cells has not been reported.

The aim of this PhD project is to investigate the activities of TCAs in breast cancer cells and evaluate potential drug repurposing for the treatment of breast-to-brain metastases.

The work will include:

  • Analysis of cell viability, cytotoxicity and apoptosis activation using the ApoTox-Glo Triplex Assay
  • Exposure of a number of breast cancer cell lines and normal breast epithelial primary cultures to amitriptyline, nortriptyline and norclomipramine (a metabolic product of clomipramine in the brain) followed
  • Drug activity in each cell line will be assessed by the inhibitory concentration 50 (IC50) value
  • establishing the possible impact of TCAs on mitochondria, through the analysis of activity in individual mitochondrial respiratory chain complexes, mitochondrial Reactive Oxygen Species (ROS) levels and membrane potential
  • The investigation of of mitochondrial DNA (mtDNA) variations as potential biomarkers to assess drug sensitivity

Funding

Self-funded PhD students only. PhD full-time and part-time courses are eligible for the Government Doctoral Loan.

2019/2020 entry

Home/EU/CI full-time students: £4,260 p/a*
Home/EU/CI part-time students: £2,130 p/a*
International full-time students: £15,100 p/a*
International part-time students: £7,550 p/a*

By Publication Fees 2018/2019

Members of staff: £1,550 p/a*
External candidates: £4,260 p/a*

*All fees are subject to annual increase.

Bench fees (in GBP)

Year 1: £10000

Year 2: £11000

Year 3: £9500

Total: £30500

Entry Requirements

  • A good honours degree or equivalent in a relevant subject or a master’s degree in an appropriate subject.
  • Exceptionally, equivalent professional experience and/or qualifications will be considered. All applicants are subject to interview.
  • English language proficiency at a minimum of IELTS band 6.5 with no component score below 6.0.

Make an enquiry

For administrative and admissions enquiries please contact christina.hampson@port.ac.uk

How to Apply

To start your application, or enquire further about the process involved, please contact Dr Qian An (qian.an@port.ac.uk) quoting both the project code PHBM4751019 and the project title.

You can also visit our How to Apply pages to get a better understanding of how the PhD application process works.

Interview date: TBC

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